Ancient Science of Life

ORIGINAL ARTICLE
Year
: 2018  |  Volume : 37  |  Issue : 4  |  Page : 188--199

Antidiabetic and nephroprotective potential of polyherbal self-fermented Ariṣṭa formulation: Evidence-based research


Amitabha Dey1, Satyajyoti Kanjilal2, Rajarshi Biswas1, Anjan Adhikari3, Satyabrata Mohapatra4, Deepa Gandhi3, Bibhuti Nath Bhatt3, Shiladitya Choudhuri5, Pallabi Chakraborty5, Tapas Kumar Sur6, Avinash Narwaria8, Chandra Kant Katiyar8 
1 Bioassay Laboratory Medical Research, Research and Development Centre, Healthcare Division, Emami Limited, Kolkata, West Bengal, India
2 Medical Research, Healthcare Division, Research and Development Centre, Emami Limited, Kolkata, West Bengal, India
3 Department of Pharmacology, R. G. Kar Medical College and Hospital, Kolkata, West Bengal, India
4 Department of Phytochemistry, Healthcare Division, Research and Development Centre, Emami Limited, Kolkata, West Bengal, India
5 Formulation Healthcare Division, Research and Development Centre, Emami Limited, Kolkata, West Bengal, India
6 Department of Pharmacology, Institute of Post Graduate Medical Education and Research and SSKM Hospital, Kolkata, West Bengal, India

Correspondence Address:
Dr. Satyajyoti Kanjilal
Medical Research, Healthcare Division, Research and Development Centre, Emami Limited, 13, BT Road, Kolkata - 700 056, West Bengal
India

Background: Ayurvedic Ariṣṭa preparations hold an age-old heritage in the prevention and cure of metabolic disorders and associated complications. Aim: The aim of the study was to study the antidiabetic activity of a self-fermented Ariṣṭa preparation (DB-07) and its potential in preventing or curing diabetes-induced nephropathy in experimental models. Materials and Methods: Rats with Streptozotocin-induced diabetes were used for studying the various dose effects of DB-07 (2.5, 3, and 3.5 ml/kg/day) in preventive and therapeutic treatment schedules. Metformin (500 mg/kg) was used as a standard drug. Fasting blood glucose and biochemical parameters such as blood protein, urea, uric acid, and creatinine were accessed by various biochemical tests. In vitro α-amylase and α-glucosidase inhibition assay and 2-deoxy glucose uptake assay using rat L6 myotubes were studied for determining the antidiabetic mechanism of action. Results: A dose-dependent and statistically significant effect on lowering the fasting blood glucose level was observed in both preventive and therapeutic scheduled treatments. Treatment with DB-07 significantly reversed the altered biochemical parameters and the effects were comparable to metformin-treated rats. DB-07 exhibited concentration-dependent inhibition of both α-amylase and α-glucosidase enzymes with an IC50 values of 10.93 μg/ml and 8.73 μg/ml, respectively. DB-07 also showed glucose uptake potential (ca. 70% activity at 125 μg/ml) in differentiated L6 myotubes. Conclusions: These observations have revealed the antidiabetic and nephroprotective potential of DB-07. DB-07 can be a potential herbal alternative to maintain a healthy blood glucose level and will prove useful in diabetes-associated renal complications.


How to cite this article:
Dey A, Kanjilal S, Biswas R, Adhikari A, Mohapatra S, Gandhi D, Bhatt BN, Choudhuri S, Chakraborty P, Sur TK, Narwaria A, Katiyar CK. Antidiabetic and nephroprotective potential of polyherbal self-fermented Ariṣṭa formulation: Evidence-based research.Ancient Sci Life 2018;37:188-199


How to cite this URL:
Dey A, Kanjilal S, Biswas R, Adhikari A, Mohapatra S, Gandhi D, Bhatt BN, Choudhuri S, Chakraborty P, Sur TK, Narwaria A, Katiyar CK. Antidiabetic and nephroprotective potential of polyherbal self-fermented Ariṣṭa formulation: Evidence-based research. Ancient Sci Life [serial online] 2018 [cited 2022 Jun 28 ];37:188-199
Available from: https://www.ancientscienceoflife.org/article.asp?issn=0257-7941;year=2018;volume=37;issue=4;spage=188;epage=199;aulast=Dey;type=0