Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 
Users Online: 3216 | Home Print this page Email this page Small font size Default font size Increase font size

 Table of Contents  
Year : 2018  |  Volume : 37  |  Issue : 4  |  Page : 180-187

Effect of Navayasa Lauha, an ayurvedic herbomineral formulation on experimentally-induced iron deficiency anemia

1 Department of Rasashastra and B.K, FOA, IMS, BHU, Varanasi, Uttar Pradesh, India
2 Department of Rasashastra and B.K, Government Ayurveda College, Raipur, Chattisgarh, India
3 Former Head, Department of Rasa Shastra, N. I. A, Jaipur, India
4 Former Dean, Adani Institute of Medical Sciences, Bhuj, Gujrat, India

Date of Submission09-Jan-2019
Date of Decision06-Sep-2020
Date of Acceptance21-Oct-2021
Date of Web Publication04-Jan-2022

Correspondence Address:
Dr. Namrata Joshi
Department of Rasashastra and B.K, FOA, IMS, BHU, Varanasi, Uttar Pradesh
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/asl.ASL_5_19

Rights and Permissions

Background: Navayasa lauha (NL) is a herbometallic preparation (Rasaushadhi) widely used in Ayurveda as a hematinic agent. Nutritional deficiency is a main cause of anemia worldwide. Materials and Methods: NL was trialed on nutritional iron deficiency anemia induced by rice milk diet given for 2 months in albino rats. Iron-deficient rats were then treated with NL for 45 days, and assessment was done on the basis of peripheral blood picture, blood parameters, viz., hemoglobin%, Packed cell volume (PCV), Total red blood cell (TRBC) count, mean cell volume (MCV), mean cell hemoglobin (MCH), MCH concentration serum iron, and “decrease in total iron binding capacity (TIBC).” Results: A highly significant improvement (P < 0.001) in PCV, MCV, MCH, and TRBC and significant increase in Hb% (P < 0.05) and serum iron (P < 0.01), and significant decrease in TIBC (P < 0.01) were also observed. Conclusion: Results of the present study demonstrate that NL could be a promising and safe herbomineral medicine in the treatment of iron deficiency anemia without any gastrointestinal upset, which are generally encountered with modern iron supplements. Significant increase in serum iron and significant decrease in TIBC are indicative of direct effect of trial drug on body iron store in the treatment of anemia.

Keywords: Albino rats, anemia, hematinic, iron deficiency, Lauha, milk, nutrition, rice

How to cite this article:
Joshi N, Dash MK, Dwivedi L, Khilnani G. Effect of Navayasa Lauha, an ayurvedic herbomineral formulation on experimentally-induced iron deficiency anemia. Ancient Sci Life 2018;37:180-7

How to cite this URL:
Joshi N, Dash MK, Dwivedi L, Khilnani G. Effect of Navayasa Lauha, an ayurvedic herbomineral formulation on experimentally-induced iron deficiency anemia. Ancient Sci Life [serial online] 2018 [cited 2023 Feb 6];37:180-7. Available from: https://www.ancientscienceoflife.org/text.asp?2018/37/4/180/334719

  Introduction Top

Iron deficiency has been recognized as the most common nutritional deficiency disorder of serious public health significance, given its impact on psychological, developmental, behavioral, and work performance. It is generally assumed that 50% of the cases of anemia are due to iron deficiency.[1] The World Health organization (WHO) has classified it amongst top ten-selected health risks.[2] The W.H.O. also estimates that 66%–80% of the population is suffering from Fe deficiency,[3] with 30% of population being in the developing countries, particularly in South Asia and Africa. It is the most common nutritional deficiency encountered in surveys of diverse populations in industrialized countries.[4] It is considerably higher for rural women (54%) than for urban women (46%) and so, looking towards its prevalence, it is included in national health program of India.[5]

According to modern science, the best treatment for iron deficiency is administration of iron supplementation therapy.[6] However, with conventional iron therapy, gastrointestinal distress is most prominent and is seen in 15%–20% of patients. Abdominal pain, nausea, vomiting, or constipation is often seen with iron therapy, leading to noncompliance.[7] Thus, some alternative medicine is the need of hour to combat such situation. Likewise modern science, in Ayurveda, based on the own principle of “application of similar substance always causes increase in the same substances”[8] and for pāṇḍu roga (iron deficiency anemia), Lauha (iron) is the best treatment.[9] Iron is supposed to be best among hematinic preparations[10] and thus indicated in the treatment of pāṇḍu roga (iron deficiency anemia). Many clinical and experimental studies have been undergone in this regard in which hemoglobin (Hb) concentration, and various red cell indices are taken for assessment criteria.[11],[12] However, in the present study, serum iron and total iron binding capacity (TIBC) estimation was also undergone that gives clear picture of iron in body tissue stores. Stainable iron in the tissue stores is the most sensitive laboratory indicator of mild iron deficiency and is particularly useful in differentiating iron deficiency from the anemia of chronic disorders. The percentage saturation of transferrin with iron and free erythrocyte protoporphyrin values does not become abnormal until tissue stores are depleted of iron. Subsequently, a decrease in the Hb concentration occurs because iron is unavailable for heme synthesis. Red blood cell indices do not become abnormal for several months after tissue stores are depleted of iron.[13] Furthermore, in the present study, no chemical was used to induce iron deficiency, rather nutritional deficiency was created by using milk and rice diet that contains phyates and phosphatase[14] and is devoid of iron. Therefore, in the present study, hematinic effect of traditional preparation NL was checked along with its effect on body iron store in nutritional iron deficiency.

  Materials and Methods Top

Drug preparation

Collection of raw drugs

All the raw ingredients of the compound formulation NL[15] were procured from pharmacy and authenticated in the Department of Dravyaguna, National Institute of Ayurveda, Jaipur, Rajasthan. Lauha bhasma was prepared by giving 60 puṭa (calcination process) in the decoction of triphalā, as per method of Ayurvedic Formulary of India.[16] Preparation of Lauha bhasma includes ayurvedic procedures of śodhana (purification), bhānupāka (sun drying), sthālīpāka, puṭapāka (levigation and calcination), and amṛtīkaṇana (necterazation) in the decoction of triphalā. The Lauha bhasma used in the present study was also undergone toxicity study as per the guidelines of OECD in which it was found safe for oral administration at therapeutic dose leve.[17]

Method of preparation

All herbal ingredients as mentioned in [Table 1] were made free from any foreign material and powdered separately till they can pass through the sieve size 100. Required quantity of powdered herbs were weighted separately and mixed homogenously. To it, equal amount of Lauha bhasma was added, and the mixture was once again mixed homogenously. All pharmaceutical processing were undergone in the Department of Rasa Shastra and Bhaisajya Kalpana, National Institute of Ayurveda, Jaipur [Figure 1].
Table 1: Constituents of Navayasa lauha

Click here to view
Figure 1: Ingredients of Navayasa Lauha

Click here to view

Ethical clearance

Ethical clearance was taken from Institutional Ethics Committee of S.M.S Medical College and Attached Hospitals, Jaipur, where the research was conducted.

Hematinic activity

Study design

In vivo hematinic activity of NL was determined in albino rats by induction of iron deficiency with the help of milk rice diet. The study was approved by Institutional Animal Ethical Committee. Dose of the test drug was calculated by extrapolating the human dose to animals based on the body surface area ratio by referring to the standard table of Paget and Barnes (1969).[18] Accordingly, accepted dose of NL was calculated to be 10 mg/100 g of body weight as shown in [Table 2]. The drug was suspended in honey and clarified butter,[15] administered orally through an orogastric tube as shown in [Figure 2].
Table 2: Study design for Haematenic study

Click here to view
Figure 2: Animal experimentation

Click here to view

Experimental animals

Wistar Albino rats (170–250 g) were obtained from the Animal Colony of Department of Zoology, Rajasthan University, Jaipur. The animals were maintained under standard laboratory conditions and water ad libitum. The room temperature was maintained at 25°C ± 1°C. All the experiments were performed in the morning according to the current guidelines for the care of the laboratory animals and the ethical guidelines for the investigation of experimental animals.

Induction of iron deficiency anemia

Eighteen rats of either sex were used in this experiment. The rats were divided into two groups containing six animals in control group receiving normal diet and Group B containing 12 animals fed on milk rice diet (depletion study) for 2 months. On completion of iron depletion study, 12 rats of Group B showing iron deficiency as evident in hematological and biochemical investigation were again divided into two groups of six rats each. One group containing 6 rats were fed with rice milk diet while in another group of 6 animals with rice milk diet NL was administered at the dose of 10mg/kg of body weight for a period of 45 days. On the day of commencement of study and at the end of the study, blood was drawn from retro bulbar space and was investigated for various assessment parameters.[19]

Parameters for assessment

In the present study, evaluation was done based on the following criteria

  • Gross behavior
  • Hematological parameters: Hb, hematocrit, mean cell volume (MCV), mean cell hemoglobin (MCH), mean cell hemoglobin concentration (MCHC), red blood cell count (RBC), and white blood cell count were performed using ABX PENTRA ML hematology analyzer[20] (HORIBA ABX SAS, France)
  • Biochemical parameters, viz., serum iron, TIBC were estimated by ferrozine method[21]
  • Morphological classification of anemia was done using microscopic examination of red blood cell morphology and values of red cell indices.

Method adopted for estimating serum total iron binding capacity and serum iron

Ferrozine method was used in the present study for estimation serum TIBC and serum iron. Serum proteins are precipitated with a reagent containing HCl (to dissociate iron), triglycolic acid (to precipitate proteins). The iron in ferrous state reacts with chromogen, to give pink-colored complex which is measured calorimetrically. For TIBC determination, serum is treated with iron standard and excess of iron is removed by adsorption of magnesium carbonate. The iron content of this serum is a measure of TIBC.

Statistical analysis

All the values were expressed as mean ± standard error of the mean. The data were analyzed by unpaired' test of P < 0.001, and P < 0.05 and 0.01 were considered as statistically highly significant and significant, respectively. Level of significance was noted and interpreted accordingly.

  Results Top

A nutritional iron deficiency by means of administration of rice and milk diet containing inhibitory substances for iron absorption, viz., phytic acid and phosphatase, was introduced in the experimental animals and the resultant effect was assessed in comparison to the rats receiving normal diet. After induction of iron-deficient diet, peripheral blood smears were taken which were suggestive of mild-to-moderate anemia [Figure 2]. Highly significant decrease in Hb% (21.62%, P < 0.001), PCV (5.91%, P < 0.001), MCH (21.39%, P < 0.001) as well as significant decrease in TRBC (7.20%, P < 0.05), MCV (6.68%, P < 0.05), MCHC (16.92%, P < 0.05), and PCV (5.91%, P < 0.001) count was clearly evident by [Table 3]. It also causes decrease in total iron level (8.22%, P < 0.05), as well as increase in TIBC (25.62%, P < 0.05), to a significant level [Table 3] and [Figure 3].
Table 3: Changes in Hematological and Biochemical Parameters after induction of Iron deficiency Anaemia in albino rats

Click here to view
Figure 3: Change in laboratory findings in iron depletion (control vs. iron deficient diet) and repletion therapy (iron deficient diet vs. Navayasa Lauha)

Click here to view

On treating iron-deficient rats with test drug, i.e., Navayasa lauha (NL), maintaining them at iron-deficient diet, exert highly significant increase in MCV (10.94%, P < 0.001); MCH (25.21%, P < 0.001); PCV (18.11% P < 0.001); and TRBC (11.06%, P < 0.001) level while significant increase in Hb% (24.24%, P < 0.05) and MCHC (16.92, P < 0.01) was found as evident in [Table 3]. Similarly, the indicators of body iron stores, viz., serum total iron (2.36%, P < 0.01) and TIBC (21.54%, P < 0.01) were also found to be altered significantly in such animals [Table 4] and [Figure 3].
Table 4: Changes in Hematological and Biochemical parameters after treatment of albino rats suffering from Iron deficiency Anaemia with Navayasa Lauha

Click here to view

  Discussion Top

NL is selected for this study as it one the most commonly used hematinic herbomineral formulation in tradition which does not imparts side effects as experienced in conventional iron therapy. Moreover, the ingredients present in it work on the principle of sāmānya pratyayārabdhatā which retains its pañcabhautika composition throughout the digestion and metabolism. Further, the ingredients present in this formulation helps in correction of nutritional iron deficiency directly as a hemetinic agent or indirectly by correction of Agni and thereby correction of all attributes of normal agni (digestive/metabolic factors) including bala (strength/physical endurance); varṇa (complexion); utsāha (enthusiasm); oja (immunity/vital energy); prabhā (glow).[22]

Being a nutritional disorder, some dietary substances are proved to induce iron deficiency in the body owing to the presence of certain inhibitory factors that make insoluble complexes with Iron, the key element involved in the genesis of the disease. This fact was utilized here in the present study, and instead of using chemicals for inducing iron deficiency, a nutritional deficiency was created by administration of milk and rice diet that contains inhibitory factors, viz., phosphatase and phyates,[7],[23] respectively. Further, the iron content of milk is very low (0.06%),[24] and on the other hand, it is a rich source of calcium. Calcium is a proven source that hinders the absorption of iron.[25] Hence, in this study, milk and rice diet was given to the experimental animal's, viz., rats, most suitable animal for conducting iron depletion–repletion therapy of iron.[14] From this study, it can be presumed that prolong nutritional deficiency (2 months in the trialed study) can gradually deplete the iron status as well as bioavailability in the body tissue as evident by decrease hemoglobin concentration and fall in red cell indices. Furthermore, decrease in functional activities as evident by sluggish movement, excess of sleep, etc., were itself suggestive of decrease in endurance activity due to some sort of nutritional deficiency. Transferrin is a protein in the blood that transports iron throughout your body and TIBC measures how well the transferrin proteins are transporting iron around your body. By examining how much iron is in the transferrin proteins, one can easily assess about concentration of iron in the blood.[26] Thus, the elevation of decreased iron as well as decrease in TIBC in rice milk-induced nutritional iron-deficient rats by administration of NL may be attributed to either change in red cell indices or replenishment of body iron store. The composition of any formulation depends on two major criteria:[27] chief constituent and supporting constituents. The chief constituent (drug) of the trial drug NL is Lauha bhasma which is a well-known hematinic agent. Supporting constituents are the drug that supports the chief ingredient in its action, drugs that eradicates the toxicity of the formulation or drugs providing media for easy transport and bioavailability in the body, i.e. vehicle. Pippali,[28] marica,[29] śuṇṭhī,[30] Mustaka,[31] viḍaṅga,[32] and citraka[33] have dīpana (stomachic), pācana (digestive), rocana (appetizer), kṛmināśaka (anti helminthic),[34] sthūlahara (analgesic),[35] hṛdya (cardiac tonic) properties. All these herbs acts as adjuvant drugs in this particular formulation [Table 5]. āmalakī,[36] harītakī[37] śuṇṭhī,[38],[39] citraka[40] which are also included in adjuvant drugs are known for their pāṇḍuhara (hematinic) effect.[41] Drugs, i.e., āmalakī,[36] harītakī,[37] and vibhītaka[42] (triphalā) viḍaṅga śuṇṭhī possessing an Anulomana (carminative) property counteract the constipative effect of lauha. Similarly, trikaṭu, viḍaṅga, triphalā, mustā, Madhu,[43],[44],[45] and ghṛta[46],[47],[48] aid in removing the metallic characters of lauha owing to their viṣaghna (antitoxic) and tridoṣahara properties and hence the ill effect of Lauha.[49] Triphalā, especially āmalakī, contains ascorbic acid which is a well-known agent that facilitates absorption of Iron.[50] Honey is also rich in copper, which helps in iron absorption.[51] Madhu[52],[53],[54] and ghṛta given as anupāna (vehicle) bring about quick action due to their yogavāhi (super-advenient) property and thereby increase the bioavailability of the formulation. Madhu and ghṛta are used as vehicle (anupāna) which is additionally beneficial in maintenance of sexual vigor (vṛṣaḥ), physical and mental stability and steadiness (Sthiratva), peacefulness and eradication of diseases (vṛṣaḥ), balance in mind and body (Samah), and longevity (śatam jīvati) with restriction graying of hair.[55]
Table 5: Therapeutic action of constituents ingredients of Navayasa Lauha

Click here to view

Moreover, the herbs present in this combination have been proven for their antioxidant,[56],[57],[58] bioavailability enhancing,[59],[60],[61] adaptogenic,[62] endurance promoting, and anti stress[63] activities and thus helps in reliving the symptomatology of the disease. Abnormal function of Agni is the root cause of all diseases. Based on this fact the constituent drugs help in breaking the pathogenesis of the pāṇḍu roga (I.D.A.) with principal of “application of similar substance always cause increase in the same substances” and “Lauha” is the best treatment imparts hematinic potential at varying levels.

  Conclusion Top

In this study, NL was found to be effective in correction of laboratory findings of iron deficiency anemia caused due to nutritional deficiency. Objective parameters including Hb%, RBC, HCT, MCV, MCHC, TIBC, and serum iron were significantly altered in experimental animals. As no adverse drug reaction has been reported in this study, it may be used as Iron supplement therapy in case of iron deficiency anemia.

Financial support and sponsorship


Conflicts of interest

There are no conflicts of interest.

  References Top

Available from: http://www.nfhsindia.org/data/india/indch7.pdf; 247. [Last accessed on 2018 Feb 02].  Back to cited text no. 1
Shaban L, Al-Taiar A, Rahman A, Al-Sabah R, Mojiminiyi O. Anemia and its associated factors among adolescents in Kuwait. Sci Rep 2020;10:5857.  Back to cited text no. 2
World Health Organization. Archived: Iron deficiency anaemia: Assessment, prevention and control; 2001. p. 114. https://www.who.int/nutrition/publications/micronutrients/anaemia_iron_deficiency/WHO_NHD_01.3/en/. [Last accessed on 2020 Sep 04].  Back to cited text no. 3
Cook JD, Finch CA, Smith NJ. Evaluation of the iron status of a population. Blood 1976;48:449-55.  Back to cited text no. 4
Available from: http://www.ncbi.nlm.nih.gov./pubmed. [Last accessed on 2018 Feb 02].  Back to cited text no. 5
Laurence and Brunton, editor. Goodman and Gilman's, the Pharmacological Basis of Therapeutics. 11th ed. California, USA: Laurence & Brunton; 2006.  Back to cited text no. 6
Kasper DL, Fauci AS, Longo DL, Braunwald E, Sauser SL, Jameson JL, editor. Harrison's Principles of Internal Medicine. 16th ed. Vol.1. New Delhi: McGraw Hill Medical Publishing Division; 2005.  Back to cited text no. 7
Tripathi B, editor. Charaka Samhita, Sutra Sthana 1/44. Varanasi: Chaukhambha Surbharti Prakashana; 1995.  Back to cited text no. 8
Vagbhata, Ashtanga Hridaya. Uttrasthana 40/49. Vidyotini Bhasa Tika by Kaviraj Atridev Gupta & Yadunandan Upadhyaya. Varanasi: Chaukhamba Sanskrit Samsthan; 2000.  Back to cited text no. 9
Karelia BN, Buch JG. Analysis of hematinic formulations available in the Indian market. J Pharmacol Pharmacother 2012;3:35-8.  Back to cited text no. 10
[PUBMED]  [Full text]  
Kumar A, Garai AK. A clinical study on Pandu Roga, iron deficiency anemia, with Trikatrayadi Lauha suspension in children. J Ayurveda Integr Med. 2012;3:215-22.   Back to cited text no. 11
Sharma S, Sharma O, Meena B, Kumavat V. Clinical efficacy and safety of Navayasa Churna in the management of iron deficiency anemia. J Res Ayurvedic Sci 2017;1:117-24.  Back to cited text no. 12
Abbaspour N, Hurrell R, Kelishadi R. Review on iron and its importance for human health. J Res Med Sci 2014;19:164-74.  Back to cited text no. 13
Hallberg L, Brune M, Rossander L. Iron absorption in man: Ascorbic acid and dose-dependent inhibition by phytate. Am J Clin Nutr 1989;49:140-4.  Back to cited text no. 14
Anonymous. Ayurvedic Formulary of India, Part-II. 17:2. 1st ed. New Delhi: Ministry of Health of Family Welfare, Govt. of India; 2003. p. 303.  Back to cited text no. 15
Anonymous. Ayurvedic Formulary of India, Part-I, 18:14. 1st ed. New Delhi: Ministry of Health of Family Welfare, Govt. of India; 2000. p. 189.  Back to cited text no. 16
Joshi N, Dash MK, Dwivedi L, Khilnani GD. Toxicity study of Lauha Bhasma (calcined iron) in albino rats. Anc Sci Life 2016;35:159-66.  Back to cited text no. 17
Paget GE, Barnes JM, Evaluation of drug activities: Pharmacometrics. In: Lawrence DR, Bacharach AL, editors. Vol. 1. New York: Academic Press; 1969. p. 161.  Back to cited text no. 18
Heimann M, Käsermann HP, Pfister R, Roth DR, Bürki K. Blood collection from the sublingual vein in mice and hamsters: A suitable alternative to retrobulbar technique that provides large volumes and minimizes tissue damage. Lab Anim 2009;43:255-60.  Back to cited text no. 19
Oliveira LR, Simionatto M, Cruz BR, Bittencourt JI, Krum EA, Moss MF, et al. Stability of complete blood count in different storage conditions using the ABX PENTRA 60 analyzer. Int J Lab Hematol 2018;40:359-65.  Back to cited text no. 20
Stookey LL. Ferrozine a new spectrophotometric re- agent for iron determination. Anal Chem 1970;42:779-84.  Back to cited text no. 21
Tripathi B, editor. Charaka, Charaka Samhita, Chkitsa Sthana 15/3-4. 5th ed. Varanasi: Chaukhambha Surbharti Prakashana; 1998. p. 550.  Back to cited text no. 22
Brune M, Rossander-Hultén L, Hallberg L, Gleerup A, Sandberg AS. Iron absorption from bread in humans: Inhibiting effects of cereal fiber, phytate and inositol phosphates with different numbers of phosphate groups. J Nutr 1992;122:442-9.  Back to cited text no. 23
Murray MJ, Murray AB, Murray NJ, Murray MB. The effect of iron status of Nigerien mothers on that of their infants at birth and 6 months, and on the concentration of Fe in breast milk. Br J Nutr 1978;39:627-30.  Back to cited text no. 24
Hallberg L, Rossander-Hultén L, Brune M, Gleerup A. Calcium and iron absorption: Mechanism of action and nutritional importance. Eur J Clin Nutr 1992;46:317-27.  Back to cited text no. 25
Available from: https://www.healthline.com ' health ' serum iron. [Last accessed on 2019 Sep 09].   Back to cited text no. 26
Kaviraj Atridev Gupta & Yadunandan Upadhyaya, Ashtanga Hridaya, Vidyotini Bhasa Tika, Uttar Sthan, 13th ed. Ver. 40/49. Varanasi: Chaukhamba Sanskrit Samsthan; 2000. p. 611.  Back to cited text no. 27
Anonymous. The Ayurvedic Pharmacopeia of India, Part-I. Vol-IV. 1st ed. New Delhi: Ministry of Health of Family Welfare; Govt. of India; 2004. p. 92.  Back to cited text no. 28
Anonymous. The Ayurvedic Pharmacopeia of India, Part-I. Vol-III. 1st ed. New Delhi: Ministry of Health of Family Welfare; Govt. of India; 1989. p. 117.  Back to cited text no. 29
Anonymous. The Ayurvedic Pharmacopeia of India, Part-I. Vol-I. 1st ed. New Delhi: Ministry of Health of Family Welfare; Govt. of India, 1989. p. 103-4.  Back to cited text no. 30
Anonymous. The Ayurvedic Pharmacopeia of India, Part-I. Vol-III. 1st ed. New Delhi: Ministry of Health of Family Welfare; Govt. of India; 1989. p. 130.  Back to cited text no. 31
Anonymous. The Ayurvedic Pharmacopeia of India, Part-I. Vol-I. 1st ed. New Delhi: Ministry of Health of Family Welfare; Govt. of India; 1989. p. 124.  Back to cited text no. 32
Anonymous. The Ayurvedic Pharmacopeia of India, Part-I. Vol-I. 1st ed. New Delhi: Ministry of Health of Family Welfare; Govt. of India; 1989. p. 29.  Back to cited text no. 33
Debebe Y, Tefera M, Mekonnen W, Abebe D, Woldekidan S, Abebe A, et al. Evaluation of anthelmintic potential of the Ethiopian medicinal plant Embelia schimperi Vatke in vivo and in vitro against some intestinal parasites. BMC Complement Altern Med 2015;15:187.  Back to cited text no. 34
Sireeratawong S, Itharat A, Lerdvuthisopon N, Piyabhan P, Khonsung P, Boonraeng S, et al. Anti-inflammatory, analgesic, and antipyretic activities of the ethanol extract of piper interruptum opiz. and Piper chaba Linn. ISRN Pharmacol 2012;2012:480265.  Back to cited text no. 35
Anonymous. The Ayurvedic Pharmacopeia of India, Part-I. Vol-I. 1st ed. New Delhi: Ministry of Health of Family Welfare; Govt. of India; 1989. p. 4.  Back to cited text no. 36
Anonymous. The Ayurvedic Pharmacopeia of India, Part-I. Vol-I. 1st ed. New Delhi: Ministry of Health of Family Welfare; Govt. of India; 1989. p. 48.  Back to cited text no. 37
Sharma PV. Dhanvantarai Nighantu. 1st ed. Ver. 2/83. Varanasi: Chaukhambha Orientaila; 1982. p. 85.  Back to cited text no. 38
Mishra Brahmashankar, Vaisya Rupalalji, Bhavprakash, Hindi Commentary, Part -1, Haritkyadi Varga. Ver. 1/150. Varanasi: Chaukhambha Sanskrit Bhawan; 2012. p. 14.  Back to cited text no. 39
Sharma PV. Dhanvantarai Nighantu. 1st ed. Ver. 2/81. Varanasi: Chaukhambha Orientaila; 1982. p. 85.  Back to cited text no. 40
Kumar A, Garai AK. A clinical study on pandu roga, iron deficiency anemia, with Trikatrayadi Lauha suspension in children. J Ayurveda Integr Med 2012;3:215-22.  Back to cited text no. 41
[PUBMED]  [Full text]  
Anonymous. The Ayurvedic Pharmacopeia of India, Part-I. Vol-I. 1st ed. New Delhi: Ministry of Health of Family Welfare; Govt. of India; 1989. p. 26.  Back to cited text no. 42
Shastri SN. Charak Samhita, Hindi Commentary, Purvardha, ed. Ver. 27/246-247. Varanasi: Chaukambha Bharti Acaedemy; 2011. p. 554.  Back to cited text no. 43
Sharma PV. Kaideva Nighantu, Pathayapayhyavibhodaka, Reprint ed. Ver. 1/177. Varanasi: Chaukhambha Orientalia; Aushadhi Varga; 2009. p. 37.  Back to cited text no. 44
Shastri AD. Sushruta Samhita, Hindi Commentary, Purvardha, Reprint edition. Ver. 45/132. Varanasi: Chaukhambha Sanskrit Saansthan; 2012. p. 232.  Back to cited text no. 45
Shastri SN, Charak Samhita, Hindi Commentary, Purvardha, ed. Ver. 27/231. Varanasi: Chaukambha Bharti acaedemy; 2011. p. 552.  Back to cited text no. 46
Sharma PV, Kaideva Nighantu, Pathayapayhyavibhodaka, Reprint ed. Ver. 4/267. Varanasi: Chaukhambha Orientalia; 2009. p. 368.  Back to cited text no. 47
Shastari Laxmipati, Yogaratnakar, Hindi Commentary Edited by Bhrama Shankar Shastri. Ver. 2. Varanasi: Chaukhambha Prakashana; 2012. p. 105.  Back to cited text no. 48
Kulkarni Dattatrreya Anant, Rasa Ratna Samucchhya, Vigyanbodhini Hindi Commentary, Reprint edition. Ver. 5/104. Delhi: Meharchand Lachhmandas Publication; 2010. p. 114.  Back to cited text no. 49
Cook JD, Reddy MB. Effect of ascorbic acid intake on nonheme-iron absorption from a complete diet. Am J Clin Nutr 2001;73:93-8.  Back to cited text no. 50
Loveridge J. The Chemistry of Bees; 2001. Available from: http://www.chm.bris.ac.uk/webprojects2001/loveridge/index-page3.html. [Last accessed on 2010 Feb 12].  Back to cited text no. 51
Pandey Gyanendra, Madanpal Nighantu, Hindi Commentary. Ver. 9/27. 1st ed. Varanasi: Chaukhambha Orientalia; Ikshvadi Varga; 2012. p. 614.  Back to cited text no. 52
Shastri SN. Charak Samhita, Hindi commentary, Purvardha, ed. Ver. 27/249. Varanasi: Chaukambha Bharti Acaedemy; 2011.  Back to cited text no. 53
Shastri AD. Sushruta Samhita, Hindi Commentary, Purvardha, Reprint edition. Ver. 45/142. Varanasi: Chaukhambha Sanskrit Sansthan; 2012. p. 233.  Back to cited text no. 54
Vagbhata, Ashtanga Hridayam, with the Commentaries, 'Sarvangasundara' of Arunadatta and 'Ayurvedarasayana' of Hemadri Collated by Dr. Anna Moreshvara Kunte, and Krishna Ramachandra Shastri Navre, edited by Pt. Harishastri Paradakar Vaidya, Gopal Mandir Lane, Uttara Stana. Ch. 39/159. Varanasi: Krishanadas Academy; 2000. p. 937.  Back to cited text no. 55
Pirzada AM, Ali HH, Naeem M, Latif M, Bukhari AH, Tanveer A. Cyperus rotundus L.: Traditional uses, phytochemistry, and pharmacological activities. J Ethnopharmacol 2015;174:540-60.  Back to cited text no. 56
Pandit S, Biswas TK, Debnath PK, Saha AV, Chowdhury U, Shaw BP, et al. Chemical and pharmacological evaluation of different ayurvedic preparations of iron. J Ethnopharmacol 1999;65:149-56.  Back to cited text no. 57
Niwano Y, Saito K, Yoshizaki F, Kohno M, Ozawa T. Extensive screening for herbal extracts with potent antioxidant properties. J Clin Biochem Nutr 2011;48:78-84.  Back to cited text no. 58
Atal CK, Zutshi U, Rao PG. Scientific evidence on the role of Ayurvedic herbals on bioavailability of drugs. J Ethnopharmacol 1981;4:229-32.  Back to cited text no. 59
Khajuria A, Zutshi U, Bedi KL. Permeability characteristics of piperine on oral absorption–an active alkaloid from peppers and a bioavailability enhancer. Indian J Exp Biol 1998;36:46-50.  Back to cited text no. 60
Johri RK, Zutshi U. An Ayurvedic formulation 'Trikatu' and its constituents. J Ethnopharmacol 1992;37:85-91.  Back to cited text no. 61
Yazdanparast R, Ardestani A. In vitro antioxidant and free radical scavenging activity of Cyperus rotundus. J Med Food 2007;10:667-74.  Back to cited text no. 62
Chen CK, Muhamad AS, Ooi FK. Herbs in exercise and sports. J Physiol Anthropol. 2012;8;31:4.  Back to cited text no. 63


  [Figure 1], [Figure 2], [Figure 3]

  [Table 1], [Table 2], [Table 3], [Table 4], [Table 5]


Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
Access Statistics
Email Alert *
Add to My List *
* Registration required (free)

  In this article
Materials and Me...
Article Figures
Article Tables

 Article Access Statistics
    PDF Downloaded150    
    Comments [Add]    

Recommend this journal