| ORIGINAL ARTICLE |
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| Year : 2017 | Volume
: 36
| Issue : 4 | Page : 187-190 |
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Determination of antimicrobial potential of five herbs used in ayurveda practices against Candida albicans, Candida parapsilosis and methicillin resistant Staphylococcus aureus
TDCP Gunasekara1, NDM Radhika2, KK Ragunathan2, DPP Gunathilaka2, MM Weerasekera1, HGSP Hewageegana3, LADM Arawwawala4, SSN Fernando1
1 Department of Microbiology, Faculty of Medical Sciences, University of Sri Jayawardenepura, Nugegoda, Sri Lanka
2 Medical Laboratory Science Unit, Faculty of Medical Sciences, University of Sri Jayawardenepura, Nugegoda, Sri Lanka
3 Department of Nidana Chikitsa, Institute of Indigenous Medicine, University of Colombo, Colombo, Sri Lanka
4 Herbal Technology Section, Industrial Technology Institute, Colombo, Sri Lanka
Correspondence Address:
TDCP Gunasekara
Department of Microbiology, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda
Sri Lanka
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/0257-7941.219360
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Background: Medicinal plants are an important source of novel antimicrobial agents. Ayurvedic treatment involves the use of a variety of medicinal plants that merit investigation. Aims: To investigate the antimicrobial activity of bark of Pongamia pinnata (L.) Pierre, stem of Rubia cordifolia Linn, leaves of Jasminum officinale Linn, stem of Berberis ceylanica C.K. Schneid. and fruit of Garcina zeylanica Roxb. Subjects and Methods: Aqueous and ethanolic extracts of dried bark of Pongamia pinnata (Magul karanda), dried stem of Rubia cordifolia Linn (Welmadata), tender leaves of Jasminum officinale Linn (Jasmine) and dried stem of Berberis ceylanica (Dāruharidrā) were prepared according to standard protocols and tested for antimicrobial activity against five clinical isolates and one standard strain each of Candida albicans (ATCC 10231), Candida parapsilosis ( ATCC 22019) and six Methicillin Resistant Staphylococcus aureus (MRSA) clinical isolates using the well diffusion method. Experiments were done in triplicates using well diffusion method. The plant extracts which gave a zone of inhibition in the well diffusion assay were further tested for Minimum Inhibitory Concentrations (MIC). Results: Aqueous and ethanolic extracts of Berberis ceylanica and ethanolic extract of Rubia cordifolia had antimicrobial activity against Candida albicans and Candida parapsilosis. Aqueous and ethanolic extracts of Garcinia zeylanica, and the ethanolic extracts of Jasminum officinale, Rubia cordifolia and Pongamia pinnata had antimicrobial activity against MRSA. Conclusions: Berberis ceylanica and Rubia crodifolia had antimicrobial activity against Candida species while Garcinia zeylanica, Jasminum officinale, Rubia crodifolia and Pongamia pinnata had antimicrobial activity against MRSA.
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