Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 
Users Online: 686 | Home Print this page Email this page Small font size Default font size Increase font size
Year : 2014  |  Volume : 34  |  Issue : 1  |  Page : 16-22

Phytochemical analysis and a study on the antiestrogenic antifertility effect of leaves of Piper betel in female albino rat

Department of Pharmacology, VSS Medical College and Hospital, Burla, Odisha, India

Correspondence Address:
Sasmita Biswal
Department of Pharmacology, VSS Medical College and Hospital, Burla, Odisha
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0257-7941.150770

Rights and Permissions

Objective: To study the effect of graded doses of the aqueous and methanolic extract of the leaves of Piper betel (PB) Linn (PBL) on the estrous cycle of female albino rats. Materials and Methods: Both the extracts were tested for their effect on the estrous cycle at three dose levels of 500, 1000 and 1500 mg/kg/day and the vaginal smears were examined daily microscopically for the different phases of the estrous cycle for a period of 30 days. Result: The estrous cycle was irregular and prolonged in the treated groups indicating anestrus condition, which would result in infertility. Both types of the extract showed a significant decrease in the duration of proestrus and estrus with a prolonged diestrus at 1000 mg/kg/day and 1500 mg/kg/day doses as compared with control. However, no change was seen in the metestrus phase. The rats treated with PB showed a significant (P < 0.05), dose-dependent decrease in the estrus phase, in comparison to the control group, the effect was more with the methanolic extract. Large, cornified cells appeared after proestrus phase with decreased number of cornified cells. There was a significant reduction in the number of the estrous cycle, in the PBL treated group. Anestrus phase appeared in all the rats treated with the aqueous and methanolic PB extract, which was not observed in the control group. However, the aqueous extract at a dose of 500 mg/kg/day had no effect either on the estrous cycle or on its different phases. The observed effect of PB leaves could be due to the flavonoids and saponin contents, which also contributes to its antiestrogenic mechanism of action. Conclusion: Both the aqueous and methanolic extract of PBL possesses antifertility effect in female albino rats.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded164    
    Comments [Add]    
    Cited by others 9    

Recommend this journal