Amelioration of oxidative stress by Tabernamontana divaricataon alloxan-induced diabetic rats
SK Kanthlal1, B Anil Kumar1, Jipnomon Joseph1, R Aravind2, P Royal Frank3
1 Department of Pharmacology, Amrita School of Pharmacy, Amrita Viswa Vidyapeetham University, AIMS, Kochi, Kerala, India
2 Department of Pharmacognosy, Amrita School of Pharmacy, Amrita Viswa Vidyapeetham University, AIMS, Kochi, Kerala, India
3 Department of Pharmacology, Erode College of Pharmacy, Erode, Tamil Nadu, India
P Royal Frank
Department of Pharmacology, Erode College of Pharmacy, Erode, Tamil Nadu
S K Kanthlal
Department of Pharmacology, Amrita School of Pharmacy, Amrita Viswa Vidyapeetham University, AIMS, Kochi, Kerala - 682 041
Source of Support: None, Conflict of Interest: None
Objective: The purpose of this study was to evaluate the anti-diabetic activity of ethanol extract of Tabernamontana divaricata (L.) and its ameliorative effect on oxidative stress in alloxan-induced diabetic rats.
Materials and Methods: Diabetes was induced by single intraperitoneal injection of alloxan monohydrate (140 mg/kg body weight). Methanol extract of T. divaricata was administered at the doses of 100 and 200 mg/kg body weight in diabetic induced rats including glibenclamide (3 mg/kg) as a reference drug. In the continuous 21 days treatment, fasting blood glucose level was determined on 0, 7, 14 and 21 days. On day 21, serum lipid profiles and glycosylated hemoglobin, liver antioxidant enzymes levels were estimated.
Results: Experimental findings showed a significant anti-diabetic potential of the extract in terms of reduction in blood glucose levels and a correct effect on the altered biochemical parameters. Observed data were found statistically significant in correction of antioxidant enzyme level accompanied with diabetes, particularly at the dose of 200 mg/kg body weight.
Conclusion: Based on the results, it can be concluded that the T. divaricata is found to be effective in type 2 diabetes in rats and to have an ameliorative effect on the associated oxidative stress.