Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 
Users Online: 119 | Home Print this page Email this page Small font size Default font size Increase font size
Year : 2013  |  Volume : 33  |  Issue : 2  |  Page : 81-84

In vitro cytotoxic activity of menispermaceae plants against HeLa cell line

1 Department of Pharmacognosy, PSG College of Pharmacy, Peelamedu, Coimbatore, India
2 Department of Pharmacognosy,Malik Deenar College of Pharmacy, Kasargod, Kerala, India
3 Department of Phytopharmacy and Phytomedicine, JSS College of Pharmacy (JSS University, Mysore), Ooty, Tamil Nadu, India

Correspondence Address:
B Samuel Thavamani
Associate Professor, Department of Pharmacognosy, PSG College of Pharmacy, Peelamedu,Coimbatore 641 004, Tamil Nadu
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0257-7941.139040

Rights and Permissions

Background: Menispermaceae, a family of flowering plants, is a medium-sized family of 70 genera totaling 420 extant species, mostly of climbing plants. It has various medicinal properties, which are used in the Ayurvedic system of medicine. Plants belonging to this family are rich in alkaloids, especially bisbenzylisoquinoline type. The hypothesis of this study is that the bisbenzylisoquinoline alkaloids present in the selected plants may exhibit in vitro cytotoxic property. Aim: The present study is aimed at estimating the total alkaloidal content of methanolic extract of Cocculus hirsutus and Cissampelos pareira and evaluating the in vitro cytotoxic activity of both the extracts on the HeLa cell line. Settings and Design: Methanolic extracts of both the plants in the concentrations of 500, 250, 125, 62.5, and 31.25 μg/ml were assessed for its cytotoxic activity by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Materials and Methods: Total alkaloidal content was studied for both the plants using ultraviolet-visible spectroscopy method. Methanol extracts of both the plants were tested for its inhibitory effect on HeLa cell line. Cytotoxicity of the plant extracts was evaluated by MTT assay. Nonlinear regression graph was plotted between % cell inhibition and Log 10 concentration, and IC 50 was determined using GraphPad Prism software. Results: Preliminary phytochemical studies confirm the presence of alkaloids in both the plants. The total alkaloids present in C. hirsutus and C. pareira were found to be 0.252%w/w and 0.1656%w/w respectively. The IC 50 values of C. hirsutus and C. pareria were found to be 111 μg/ml and 129.3 μg/ml respectively. Conclusion: From this study, it is observed that C. hirsutus and C. pareira have in vitro cytotoxic activity against HeLa cell line.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded227    
    Comments [Add]    
    Cited by others 1    

Recommend this journal