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Year : 2007  |  Volume : 27  |  Issue : 1  |  Page : 1-5

Preliminary phytochemical investigation and antibacterial activity of Phyllanthus amarus Schum & Thorn.

1 Medicinal Plants Research Unit, P.G. & Research Department of Microbiology, Thanthai Hans Roever College, Perambalur 621 212, India
2 Laboratory of Plant Science, Department of Biotechnology, K.S.R.College of Technology, Tiruchengode, India
3 Department of Biotechnology,Thanthai Hans Roever College, Perambalur 621212, India
4 Department of Botany and Microbiology, A.V.V.M. Sri Puspham College, Poondi, India

Correspondence Address:
R Dhandapani
Medicinal Plants Research Unit, P.G. & Research Department of Microbiology, Thanthai Hans Roever College, Perambalur 621 212
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Source of Support: None, Conflict of Interest: None

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The aqueous extract of Phyllanthus amarus  Schum & Thorn, was selected for preliminary phytochemical and antibacterial activity against human pathogens. Minimum inhibitory concentration was performed by broth dilution method and the zone of inhibition was studied by agar disc diffusion method at concentrations of 10 and 20 mg/ml in DMSO (Dimethyl Sulfoxide). The results revealed the antimicrobial activity of extract against the the strains of microorganisms concentrations in ranges of 60 and 500 μg/ml. Aqueous extract of leaves and roots of Phyllanthus amarus  exhibited significant antibacterial activity against eight test bacteria like Staphylococcus aureus, Bacillus subtilis, Staphylococcus albus, Streptococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Proteus vulgaris.  The plant under study revealed the presence of alkaloids, phytosterols, phenolic compounds and tannins, proteins and amino acids , lignins and saponins.

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