Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 
Users Online: 1181 | Home Print this page Email this page Small font size Default font size Increase font size
Year : 2003  |  Volume : 22  |  Issue : 4  |  Page : 134-139

In-vitro culture studies for callus and root generation of Boerhaavia diffusa Linn

1 B.R. Nahata College of Pharmacy, Mandsaur-458 001, M.P., India
2 Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur-492 010. C.G., India

Correspondence Address:
Vandana Jain
B.R. Nahata College of Pharmacy, Mandsaur-458 001, M.P.
Login to access the Email id

Source of Support: None, Conflict of Interest: None

Rights and PermissionsRights and Permissions

Leaf and stem explants excised from young plant of B.diffusa  were cultured on Murashige and Skoog (MS) medium containing agar (0.8%), sucrose (2.5%) and varied concentration of Indole butyric acid (IBA), Napthyl acetic acid (NAA), 2,4 - dichloro phenoxy acetic acid (2,4-D) and Picrolam. Leaf explants has given better response for both rooting and callus formation. IBA in a concentration of 5μM has shown maximum regeneration (69.7%) with induction period of 7 days. The developed roots were similar to that of naturally grown roots with little anatomical changes. For callus formation 20μM 2, 4-D has given maximum amount and percentage response 979.5% with an induction period of 8 days. Picrolam (10μM) has shown 36.6% response and the average weight of callus was less as compared with 2,4-D. The callus obtained was friable and opaque in nature.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded56    
    Comments [Add]    

Recommend this journal